Number 1. (in 1 part) December 28, 2014
1. Khadidja Bouzid, Fouzia Toumi Benali, Rabah Chadli, Mohamed Bouzouina, Aman Bouzid, Amal Benchohra, Mustapha Mahmoud Dif
Extraction, Identification and Quantitative HPLC Analysis of Flavonoids From Fruit Extracts of Arbutus unedo L from Tiaret Area (Western Algeria)
European Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 160-168.
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2. Ignat Ignatov, Oleg MosinEuropean Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 160-168.
Abstract:
The aim of the current study was to evaluate the total phenolic, flavonoid content and to investigate the antioxidant capacities of the fruit extracts of Arbutus unedo L. that grows in Tiaret area (Western Algeria). First we have extracted the fruit by some non-polar solvent (chloroform, ethyl acetate, 1-butanol). Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. Extracts content was determined by using a high-performance liquid chromatography (HPLC)-UV method. The total phenolic contents of A.unedo L. varied between 12.75±0.06 to 34.17±1.36 mg gallic acid equivalent/g of dry weight of extract. The total flavonoid varied from 2.18±0.10 to 6.54±1.14 mg catechin equivalent/g. The antioxidant potential of all extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, the IC50 of acetate ethyl was the best by 0,009 mg/ml may due to the phenolic compound, in the second was the chloroform extract by IC50=0,015mg/ml, in the third was butanol extract by IC50= 0,022 mg/ml and in the last was water extract by IC50= 0,048mg/ml. the antioxidant activity of all extracts was better than ascorbic acid. The extract obtained under optimum conditions was analyzed by HPLC and five flavonoid compounds were identified; they are catechin, apiginin, silybin, fisetine and naringin.
The aim of the current study was to evaluate the total phenolic, flavonoid content and to investigate the antioxidant capacities of the fruit extracts of Arbutus unedo L. that grows in Tiaret area (Western Algeria). First we have extracted the fruit by some non-polar solvent (chloroform, ethyl acetate, 1-butanol). Total phenolic content and total flavonoid content were evaluated according to the Folin-Ciocalteu procedure, and a colorimetric method, respectively. Extracts content was determined by using a high-performance liquid chromatography (HPLC)-UV method. The total phenolic contents of A.unedo L. varied between 12.75±0.06 to 34.17±1.36 mg gallic acid equivalent/g of dry weight of extract. The total flavonoid varied from 2.18±0.10 to 6.54±1.14 mg catechin equivalent/g. The antioxidant potential of all extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, the IC50 of acetate ethyl was the best by 0,009 mg/ml may due to the phenolic compound, in the second was the chloroform extract by IC50=0,015mg/ml, in the third was butanol extract by IC50= 0,022 mg/ml and in the last was water extract by IC50= 0,048mg/ml. the antioxidant activity of all extracts was better than ascorbic acid. The extract obtained under optimum conditions was analyzed by HPLC and five flavonoid compounds were identified; they are catechin, apiginin, silybin, fisetine and naringin.
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Modeling of Possible Conditions for Origin of First Organic Forms in hot Mineral Water
European Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 169-179.
Number of views: 2644 Download in PDF
3. Oleg Mosin, Ignat Ignatov, Dmitry Skladnev, Vitaly ShvetsEuropean Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 169-179.
Abstract:
The composition of water, its temperature and pH value was analyzed in experiments with modelling of primary hydrosphere and possible conditions for origin of first organic forms in hot mineral water. For this aim the authors performed experiments with hot mineral and seawater from Bulgaria by IR-spectrometry (DNES-method). As model systems were used cactus juice of Echinopsis pachanoi and Mediterranean jellyfish Cotylorhiza tuberculata. It was considered the reactions of condensation and dehydration in alkaline aqueous solutions with рН = 9–10, resulting in synthesis from separate molecules larger organic molecules as polymers and short polipeptides. It was shown that hot alkaline mineral water with temperature from +65 0C to +95 0C and pH value from 9 to 11 is more suitable for the origination of life and living matter than other analyzed water samples. The pH value of seawater on contrary is limited to the range of 7,5 to 8,4 units. Two common local maximums were observed in the IR-spectra of jellyfish and seawater, which were more pronouncedly expressed in IR-spectra of jellyfish.
The composition of water, its temperature and pH value was analyzed in experiments with modelling of primary hydrosphere and possible conditions for origin of first organic forms in hot mineral water. For this aim the authors performed experiments with hot mineral and seawater from Bulgaria by IR-spectrometry (DNES-method). As model systems were used cactus juice of Echinopsis pachanoi and Mediterranean jellyfish Cotylorhiza tuberculata. It was considered the reactions of condensation and dehydration in alkaline aqueous solutions with рН = 9–10, resulting in synthesis from separate molecules larger organic molecules as polymers and short polipeptides. It was shown that hot alkaline mineral water with temperature from +65 0C to +95 0C and pH value from 9 to 11 is more suitable for the origination of life and living matter than other analyzed water samples. The pH value of seawater on contrary is limited to the range of 7,5 to 8,4 units. Two common local maximums were observed in the IR-spectra of jellyfish and seawater, which were more pronouncedly expressed in IR-spectra of jellyfish.
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Studying of Phenomenon of Biological Adaptation to Heavy Water
European Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 180-209.
Number of views: 2552 Download in PDF
4. Valery V. Novochadov, Kristina A. Bovol’skaya, Sof’’ya A. Lipnitzkaya, Ekaterina V. Perevalova, Ekaterina Yu. Shuvalova, Zoya N. Zagrebina, Valery G. ZaytzevEuropean Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 180-209.
Abstract:
Biological influence of deuterium on cells of various taxonomic groups of prokaryotic and eucaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photo-organotrophic, and photosynthetic ways of assimilation of carbon substrates (methylotrophic bacteria Brevibacterium methylicum, chemoheterotrophic bacteria Bacillus subtilis, photo-organotrophic halobacteria Halobacterium halobium, and green micro algae Chlorella vulgaris) was investigated at the growth on media with heavy water (2H2O). For investigated microorganisms are submitted the data on growth and adaptation on the growth media containing as sources of deuterated substrates 2H2O, [2H]methanol and hydrolisates of deutero-biomass of methylotrophic bacteria B. methylicum, obtained after multistage adaptation to 2H2O. The qualitative and quantitative composition of intra- and endocellular amino acids, proteins, carbohydrates and fatty acids in conditions of adaptation to 2H2O is investigated. It is shown, that the effects observed at adaptation to 2H2O, possess a complex multifactorial character and connected to cytological, morphological and physiological changes – the magnitude of the lag- period, time of cellular generation, output of biomass, a parity ratio of synthesized amino acids, proteins, carbohydrates and lipids, and also with an evolutionary level of the organization of the investigated object and the pathways of assimilation of carbon substrates as well.
Biological influence of deuterium on cells of various taxonomic groups of prokaryotic and eucaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photo-organotrophic, and photosynthetic ways of assimilation of carbon substrates (methylotrophic bacteria Brevibacterium methylicum, chemoheterotrophic bacteria Bacillus subtilis, photo-organotrophic halobacteria Halobacterium halobium, and green micro algae Chlorella vulgaris) was investigated at the growth on media with heavy water (2H2O). For investigated microorganisms are submitted the data on growth and adaptation on the growth media containing as sources of deuterated substrates 2H2O, [2H]methanol and hydrolisates of deutero-biomass of methylotrophic bacteria B. methylicum, obtained after multistage adaptation to 2H2O. The qualitative and quantitative composition of intra- and endocellular amino acids, proteins, carbohydrates and fatty acids in conditions of adaptation to 2H2O is investigated. It is shown, that the effects observed at adaptation to 2H2O, possess a complex multifactorial character and connected to cytological, morphological and physiological changes – the magnitude of the lag- period, time of cellular generation, output of biomass, a parity ratio of synthesized amino acids, proteins, carbohydrates and lipids, and also with an evolutionary level of the organization of the investigated object and the pathways of assimilation of carbon substrates as well.
Number of views: 2552 Download in PDF
Different Phenotype of Chondrocytes in Articular Cartilage: Mapping, Possible Mechanisms, and Impact to Implant Healing
European Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 210-222.
Number of views: 2415 Download in PDF
5. Ajay Tomer, Ramji Singh, Manoj Kumar MauryaEuropean Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 210-222.
Abstract:
A remodelling of articular cartilage due to damage or pathological state development is characterized by an alteration in cartilage homeostasis including distribution of the chondrocytes. Said alteration can be determined by cellular response changes during cartilage regeneration and remodelling. However, features of cellular distrubution in native cartilage is not yet clear. This study was undertaken to select representative set of regulatory molecules and their receptors related to the formation of mosaic structures in the native cartilage. We used bioinformatical approaches and mapping of the chondrocyte phenotype markers in different areas of the intact articular cartilage. Differences in the phenotype markers expression as like as in cellular density gradient from tidemark to articular surface were observed. Additionaly, we have found specific order of lateral cellular distribution in the intact cartilage. The database of molecular processes in chondrocytes have been obtained, seems to be suitable for further investigation of the articular cartilage remodeling. The hypothetical model of scaffold to satisfy the conditions of mosaic structure formation in cartilage after substitution of full-layer defects and stimulation of colonization by cells from the bone marrow, have been proposed.
A remodelling of articular cartilage due to damage or pathological state development is characterized by an alteration in cartilage homeostasis including distribution of the chondrocytes. Said alteration can be determined by cellular response changes during cartilage regeneration and remodelling. However, features of cellular distrubution in native cartilage is not yet clear. This study was undertaken to select representative set of regulatory molecules and their receptors related to the formation of mosaic structures in the native cartilage. We used bioinformatical approaches and mapping of the chondrocyte phenotype markers in different areas of the intact articular cartilage. Differences in the phenotype markers expression as like as in cellular density gradient from tidemark to articular surface were observed. Additionaly, we have found specific order of lateral cellular distribution in the intact cartilage. The database of molecular processes in chondrocytes have been obtained, seems to be suitable for further investigation of the articular cartilage remodeling. The hypothetical model of scaffold to satisfy the conditions of mosaic structure formation in cartilage after substitution of full-layer defects and stimulation of colonization by cells from the bone marrow, have been proposed.
Number of views: 2415 Download in PDF
Determination of Effect of Substrate Concentration and Dilution of Inoculums on Population Dynamics of Pseudomonas Fluorescens
European Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 223-228.
Number of views: 2576 Download in PDF
6. European Journal of Molecular Biotechnology, 2014, Vol.(6), № 4, pp. 223-228.
Abstract:
Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flourescens when used as substrate for mass culturing of antagonist. The present investigation were undertaken to test the suitability of neem and Jatropha cakes for liquid Concentration and longevity of Pseudomonas flourescens in vitro. Increasing the dilution of stock of initial inoculums resulted in decrease of total viable counts of Pseudomonas flourescens was comparatively highest population (64.67×105) and (201.67×105) lesser after 15 days than after 7 days of inoculation on Neem and Jatropha cake concentration respectively.
Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flourescens when used as substrate for mass culturing of antagonist. The present investigation were undertaken to test the suitability of neem and Jatropha cakes for liquid Concentration and longevity of Pseudomonas flourescens in vitro. Increasing the dilution of stock of initial inoculums resulted in decrease of total viable counts of Pseudomonas flourescens was comparatively highest population (64.67×105) and (201.67×105) lesser after 15 days than after 7 days of inoculation on Neem and Jatropha cake concentration respectively.
Number of views: 2576 Download in PDF