2 December 18, 2017
Articles and Statements
1. N. Ourhriss, A. Zeroual, C. A. Gadhi, A. Benharref, A. Abourriche, A. Bennamara, A. ElHajbi
Synthesis, spectroscopic NMR and theoretical (HF and DFT) investigation of 3,5,5,9-tetramethyl-2-nitro-6,7,8,9-tetrahydro-5H-benzocycloheptene and 2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene
European Journal of Molecular Biotechnology, 2017, 5(2): 52-59.
Number of views: 1588 Download in PDF
2. I.A.A. Ibrahim, M.M. Mohammad, A.A. Faisal, H. Musa, G.Altine, A. BununuEuropean Journal of Molecular Biotechnology, 2017, 5(2): 52-59.
Abstract:
3,5,5,9-tetramethyl-2-nitro-6,7,8,9-tetrahydro-5H-benzocycloheptene and 2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene has been synthesized using nitration reaction of ar-himachalene. Also, optimized geometry of the title compound are evaluated using HF and DFT/B3LYP with 6-31(d) basis set 6-31G(d) methods. Moreover, the 13C NMR chemical shift values of the molecule (2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene) are calculated using HF and DFT/B3LYP/6-31G(d) and compared with experimental results. The global of the reactivity indices and Parr functions of the reagents were calculated by HF and DFT to identify the actives regions of these molecules, we found that these reactions are regiospecific.
3,5,5,9-tetramethyl-2-nitro-6,7,8,9-tetrahydro-5H-benzocycloheptene and 2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene has been synthesized using nitration reaction of ar-himachalene. Also, optimized geometry of the title compound are evaluated using HF and DFT/B3LYP with 6-31(d) basis set 6-31G(d) methods. Moreover, the 13C NMR chemical shift values of the molecule (2,5,9,9-tetramethyl-1,3-dinitro-6,7,8,9-tetrahydro-5H-benzocycloheptene) are calculated using HF and DFT/B3LYP/6-31G(d) and compared with experimental results. The global of the reactivity indices and Parr functions of the reagents were calculated by HF and DFT to identify the actives regions of these molecules, we found that these reactions are regiospecific.
Number of views: 1588 Download in PDF
Extraction of Alkaloids from Three Nigerian Plants, Kola Acuminata (OJI IGBO) Vera Kola (OJI Hausa) and Garcinia Kola (BITTER KOLA)
European Journal of Molecular Biotechnology, 2017, 5(2): 60-65.
Number of views: 1652 Download in PDF
3. Baigalmaa Luvsandorj, MorigenEuropean Journal of Molecular Biotechnology, 2017, 5(2): 60-65.
Abstract:
The extraction of alkaloid from Garcinia Kola (bitter kola) Kola Accuminata (Oji Igbo) and Kola Vera (Oji Hausa) were carried out using 10 % enthanoic acid and 10 % methanol to separate the alkaloid from the residue. The alkaloid was extracted using ammonium hydroxide from the sample results obtained on alkaloid for the sample 9.16 % Oji Igbo, 6.20 % Oji Hausa and 8.20 % Bitter Kola. It was observed that the percentage of alkaloids was highest in Oji Igbo and the least for Oji Hausa. The extracts all exhibited antimicrobial activity against he tested organisms. These activities decreasing with a decrease in extract concentrations. The results suggest the usefulness of thee nuts in the treatment or management of microbial infections without the dangers of side effects from synthetic drugs.
The extraction of alkaloid from Garcinia Kola (bitter kola) Kola Accuminata (Oji Igbo) and Kola Vera (Oji Hausa) were carried out using 10 % enthanoic acid and 10 % methanol to separate the alkaloid from the residue. The alkaloid was extracted using ammonium hydroxide from the sample results obtained on alkaloid for the sample 9.16 % Oji Igbo, 6.20 % Oji Hausa and 8.20 % Bitter Kola. It was observed that the percentage of alkaloids was highest in Oji Igbo and the least for Oji Hausa. The extracts all exhibited antimicrobial activity against he tested organisms. These activities decreasing with a decrease in extract concentrations. The results suggest the usefulness of thee nuts in the treatment or management of microbial infections without the dangers of side effects from synthetic drugs.
Number of views: 1652 Download in PDF
The QseB/QseC Signaling Affects Initiation of Chromosomal Replication through Regulating Expression of the DnaA Protein in E.coli
European Journal of Molecular Biotechnology, 2017, 5(2): 66-73.
Number of views: 1536 Download in PDF
4. I.A.A. Ibrahim, M. M. Mohammad, A. A. Faisal, H. Musa, G. Altine, A. BununuEuropean Journal of Molecular Biotechnology, 2017, 5(2): 66-73.
Abstract:
The Escherichia coli two-component system, QseB/QseC signaling regulates expressions of more than 50 genes encoding flagellar proteins and proteins associated with virulence. In this work, we found that absence of the qseB gene led to an early initiation of chromosomal replication in a manner of medium-independent, the early initiation was shown to be reversed by ectopic expression of QseB from a plasmid pqseB. Further, absence of the qseB gene resulted in an increase of DnaA concentration and the purified QseB protein did not interact with the oriC DNA in vitro. The early initiation was also found in the ΔqseC mutant cells. These results indicate that absence of the QseB/QseC signaling increases concentration of DnaA and the later subsequently triggers the early initiation of replication. Flourescene microscope analysis showed that the QseC protein localized at cell membrane while QseB was in cytosol.
The Escherichia coli two-component system, QseB/QseC signaling regulates expressions of more than 50 genes encoding flagellar proteins and proteins associated with virulence. In this work, we found that absence of the qseB gene led to an early initiation of chromosomal replication in a manner of medium-independent, the early initiation was shown to be reversed by ectopic expression of QseB from a plasmid pqseB. Further, absence of the qseB gene resulted in an increase of DnaA concentration and the purified QseB protein did not interact with the oriC DNA in vitro. The early initiation was also found in the ΔqseC mutant cells. These results indicate that absence of the QseB/QseC signaling increases concentration of DnaA and the later subsequently triggers the early initiation of replication. Flourescene microscope analysis showed that the QseC protein localized at cell membrane while QseB was in cytosol.
Number of views: 1536 Download in PDF
Phytochemical Analysis and Antibacterial Assay of Stem Bark of Anogeissus Leiogarpus
European Journal of Molecular Biotechnology, 2017, 5(2): 74-79.
Number of views: 1590 Download in PDF
5. Oleg V. GradovEuropean Journal of Molecular Biotechnology, 2017, 5(2): 74-79.
Abstract:
Anogeissus leiocarpus is extensively used in Nupe traditional and folklore medicine to cure various human ailments. The preliminary phytochemical screening of the stem bark revealed the presence of flavonoids, soponins, steroids, terpeniods, cardiac glycosides and anthraquinones. In vitro antibacterial studies of the methanol and water extracts of the plant part were carried out on medically important bacterial strains including, Lacbacillus spp, Staphylococcus aureus, Pseudomonas aerugininosa, Escherichia coli, Bacillus spp, and Salmonella typhii using agar plug techniques. The results compared well with standard antibiotics (streptomycin) with susceptibly increasing with concentration. The extracts of the plant showed antibacterial activity, justifying their continued used in treatment of bacterial infections.
Anogeissus leiocarpus is extensively used in Nupe traditional and folklore medicine to cure various human ailments. The preliminary phytochemical screening of the stem bark revealed the presence of flavonoids, soponins, steroids, terpeniods, cardiac glycosides and anthraquinones. In vitro antibacterial studies of the methanol and water extracts of the plant part were carried out on medically important bacterial strains including, Lacbacillus spp, Staphylococcus aureus, Pseudomonas aerugininosa, Escherichia coli, Bacillus spp, and Salmonella typhii using agar plug techniques. The results compared well with standard antibiotics (streptomycin) with susceptibly increasing with concentration. The extracts of the plant showed antibacterial activity, justifying their continued used in treatment of bacterial infections.
Number of views: 1590 Download in PDF
Autopoiesis Concepts for Chemical Origins of Life and Synthetic Biology. Stenogram of the popular lecture on the foreign bibliographic seminar
European Journal of Molecular Biotechnology, 2017, 5(2): 80-88.
Number of views: 1619 Download in PDF
6. European Journal of Molecular Biotechnology, 2017, 5(2): 80-88.
Abstract:
The monograph (Luisi P.L. "The Emergence of Life: From Chemical Origins to Synthetic Biology", 2010, Cambridge University Press, Cambridge, New York etc., 315 p.) is a well-written, informative book providing a novel view on the interrelation between the abiogenesis as the natural origin of life and synthetic biology as the artificial synthesis of life. This concept is specially known as autopoiesis. As its name implies, it is a correlate of self-organization, but this word has quite a broad meaning in the literature. Consequently, some further restriction is required for this term in abiogenetic, as well as in "biogenetic" applications. There is, in fact, one basic reason for considering the abiogenetic problem in terms of self-organization theory. It follows from the extremely boundless complexity of biological systems.
The monograph (Luisi P.L. "The Emergence of Life: From Chemical Origins to Synthetic Biology", 2010, Cambridge University Press, Cambridge, New York etc., 315 p.) is a well-written, informative book providing a novel view on the interrelation between the abiogenesis as the natural origin of life and synthetic biology as the artificial synthesis of life. This concept is specially known as autopoiesis. As its name implies, it is a correlate of self-organization, but this word has quite a broad meaning in the literature. Consequently, some further restriction is required for this term in abiogenetic, as well as in "biogenetic" applications. There is, in fact, one basic reason for considering the abiogenetic problem in terms of self-organization theory. It follows from the extremely boundless complexity of biological systems.
Number of views: 1619 Download in PDF