1 March 25, 2017
Articles and Statements
1. Alexey S. Afoshin, Philipp V. Kochetkov, Zhanna I. Andreeva-Kovalevskaya, Zhanna I. Budarina, Marina V. Zakharova, Alexander V. Lisov, Andrey M. Shadrin, Alexey A. Leontievsky
A High-Throughput PCR-Amplification of GC-Rich DNA Sequences
European Journal of Molecular Biotechnology, 2017, 5(1): 4-10.
Number of views: 1926 Download in PDF
2. Ignat IgnatovEuropean Journal of Molecular Biotechnology, 2017, 5(1): 4-10.
Abstract:
The PCR amplification of DNA molecules with a high GC content is a complex procedure and often requires optimisation. In last few years several improvements have been developed to facilitate the optimisation of PCR steps. In this work, five high-fidelity commercial DNA-polymerases for the amplification of GC-rich genes were tested. Using the most effective DNA polymerase, we were able to PCR amplify 161 of 187 (87 %) of target genes with a GC content from 57 to 77 % mostly up to 2500 bp long without an optimisation steps. The final yield of specific PCR products was more than 150 ng from 100 μL PCR reactions mix after the purification of specific DNA products by agarose gel electrophoresis.
The PCR amplification of DNA molecules with a high GC content is a complex procedure and often requires optimisation. In last few years several improvements have been developed to facilitate the optimisation of PCR steps. In this work, five high-fidelity commercial DNA-polymerases for the amplification of GC-rich genes were tested. Using the most effective DNA polymerase, we were able to PCR amplify 161 of 187 (87 %) of target genes with a GC content from 57 to 77 % mostly up to 2500 bp long without an optimisation steps. The final yield of specific PCR products was more than 150 ng from 100 μL PCR reactions mix after the purification of specific DNA products by agarose gel electrophoresis.
Number of views: 1926 Download in PDF
Distribution of Molecules of ZEOLITH detox and ZEOLITH Crème in Water as Factor for Health
European Journal of Molecular Biotechnology, 2017, 5(1): 11-22.
Number of views: 1795 Download in PDF
3. Dimitar Mehandjiev, Ignat Ignatov, Stoil Karadzhov, Georgi Gluhchev, Atanas AtanasovEuropean Journal of Molecular Biotechnology, 2017, 5(1): 11-22.
Abstract:
We studied the mathematical model of interaction with water of natural mineral and microporous crystalline mineral ZEOLITH detox and ZEOLITH Creme of LavaVitae Company (Austria). In this report are submitted data about the interaction of ZEOLITH detox and ZEOLITH Creme with water, obtained by non-equilibrium (NES) and differential-equilibrium energy spectrum (DNES) of water. The average energy (ΔEH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ΔE=-0.0034±0.0011 eV for ZEOLITH detox. The average energy (ΔEH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ΔE=-0.0034±0.0011 eV for ZEOLITH detox. The average energy (ΔEH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ΔE=-0.007±0.0011 eV for ZEOLITH Creme. These results suggest the restructuring of ΔEH... O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. The research is performed for ZEOLITH detox with study of pH and oxidative reduction potential (ORP).
We studied the mathematical model of interaction with water of natural mineral and microporous crystalline mineral ZEOLITH detox and ZEOLITH Creme of LavaVitae Company (Austria). In this report are submitted data about the interaction of ZEOLITH detox and ZEOLITH Creme with water, obtained by non-equilibrium (NES) and differential-equilibrium energy spectrum (DNES) of water. The average energy (ΔEH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ΔE=-0.0034±0.0011 eV for ZEOLITH detox. The average energy (ΔEH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ΔE=-0.0034±0.0011 eV for ZEOLITH detox. The average energy (ΔEH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of ZEOLITH detox with water measured by NES- and DNES-methods is ΔE=-0.007±0.0011 eV for ZEOLITH Creme. These results suggest the restructuring of ΔEH... O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. The research is performed for ZEOLITH detox with study of pH and oxidative reduction potential (ORP).
Number of views: 1795 Download in PDF
Processes in Catholyte and Anolyte as Result of Water Electrolysis
European Journal of Molecular Biotechnology, 2017, 5(1): 23-29.
Number of views: 1809 Download in PDF
4. H.A. Shindy, A.K. Khalafalla, M.M. Goma, A.H. EedEuropean Journal of Molecular Biotechnology, 2017, 5(1): 23-29.
Abstract:
A two stage model of the physicochemical processes at the electrolysis of pure water is proposed. The presence of nascent hydrogen in the catholyte and nascent oxygen in the anolyte during the first stage explains the antioxidant properties of the catholyte and the strong biocidal action of the anolyte. In the second stage the nascent hydrogen and oxygen are combined into hydrogen and oxygen molecules, respectively. The comparison between their average energies with the average energy of the control sample of water shows an increase in the average energy of the catholyte and decrease in the average energy of the anolyte. This indicates that some changes in the structure of the activated water have occurred.
A two stage model of the physicochemical processes at the electrolysis of pure water is proposed. The presence of nascent hydrogen in the catholyte and nascent oxygen in the anolyte during the first stage explains the antioxidant properties of the catholyte and the strong biocidal action of the anolyte. In the second stage the nascent hydrogen and oxygen are combined into hydrogen and oxygen molecules, respectively. The comparison between their average energies with the average energy of the control sample of water shows an increase in the average energy of the catholyte and decrease in the average energy of the anolyte. This indicates that some changes in the structure of the activated water have occurred.
Number of views: 1809 Download in PDF
Synthesis, Spectral Sensitization, Solvatochromic and Halochromic Evaluation of New Monomethine and Trimethine Cyanine Dyes
European Journal of Molecular Biotechnology, 2017, 5(1): 30-42.
Number of views: 1753 Download in PDF
5. A. Zeroual, M. El Idrissi, R. El Ajlaoui, N. Ourhriss, S. Abouricha, N. Mazoir, A. Benharref, A. El HajbiEuropean Journal of Molecular Biotechnology, 2017, 5(1): 30-42.
Abstract:
New heterocyclic starting material namely 4,5-dimethyl-2,7-diphenyl-furo[(3,2-d), ( 3, 2 -d)] bis pyrazole were prepared and employed to synthesis of some novel monomethine cyanine dyes (simple cyanine dyes) and trimethine cyanine dyes (carbocyanine dyes). Spectral sensitization evaluation for all the synthesized cyanine dyes was carried out through investigating their electronic visible absorption spectra in 95% ethanol solution. Solvatochromic and/or hachromic evaluation for some selected dyes were carried out through examining their electronic visible absorption spectra in pure solvents having different polarities [Water (78.54), Dimethylformamide (36.70), Ethanol (24.3), Chloroform (4.806), Carbontetrachloride (2.238) and Dioxane (2.209)] and/or in aqueous universal buffer solutions having varied pH values (1.75, 2.45, 4.65, 5.80, 7.88, 8.75, 10.85 and 12.60 units), respectively. Structure determination were carried out via elemental analysis, visible spectra. Mass spectrometer, IR and 1H NMR spectral data.
New heterocyclic starting material namely 4,5-dimethyl-2,7-diphenyl-furo[(3,2-d), ( 3, 2 -d)] bis pyrazole were prepared and employed to synthesis of some novel monomethine cyanine dyes (simple cyanine dyes) and trimethine cyanine dyes (carbocyanine dyes). Spectral sensitization evaluation for all the synthesized cyanine dyes was carried out through investigating their electronic visible absorption spectra in 95% ethanol solution. Solvatochromic and/or hachromic evaluation for some selected dyes were carried out through examining their electronic visible absorption spectra in pure solvents having different polarities [Water (78.54), Dimethylformamide (36.70), Ethanol (24.3), Chloroform (4.806), Carbontetrachloride (2.238) and Dioxane (2.209)] and/or in aqueous universal buffer solutions having varied pH values (1.75, 2.45, 4.65, 5.80, 7.88, 8.75, 10.85 and 12.60 units), respectively. Structure determination were carried out via elemental analysis, visible spectra. Mass spectrometer, IR and 1H NMR spectral data.
Number of views: 1753 Download in PDF
MEDT Study of the Mechanism and Regioselectivity of Diazocompounds and Alkenes in [3+2] Cycloaddition Reaction
European Journal of Molecular Biotechnology, 2017, 5(1): 43-49.
Number of views: 1775 Download in PDF
6. European Journal of Molecular Biotechnology, 2017, 5(1): 43-49.
Abstract:
The mechanism and regioselectivity of diazomethane with 2-methyl-but-2-ene and (diazomethylene)dibenzene with Methylene-trifluoromethyl-phosphane in [3+2] cycloaddition, have been theoretically studied at the DFT/ B3LYP/6-31(d) computational level. The possible ortho/meta regioselective channels were explored and characterized, the energies analysis associated with the different reaction pathways and the analysis of the density map of the transition indicates that the 1,3-DC reaction of diazomethane with 2-methyl-but-2-ene and (diazomethylene)dibenzene with Methylene-trifluoromethyl-phosphane are highly regioselective, in good agreement with the experimental observations.
The mechanism and regioselectivity of diazomethane with 2-methyl-but-2-ene and (diazomethylene)dibenzene with Methylene-trifluoromethyl-phosphane in [3+2] cycloaddition, have been theoretically studied at the DFT/ B3LYP/6-31(d) computational level. The possible ortho/meta regioselective channels were explored and characterized, the energies analysis associated with the different reaction pathways and the analysis of the density map of the transition indicates that the 1,3-DC reaction of diazomethane with 2-methyl-but-2-ene and (diazomethylene)dibenzene with Methylene-trifluoromethyl-phosphane are highly regioselective, in good agreement with the experimental observations.
Number of views: 1775 Download in PDF