Number 1. (in 1 part) September 15, 2013
1. Nurshat M. Gaifullin, Alexey V. Bachurin
Recombinant Bone Morphogenetic Protein 2 Stimulates the Remodeling Chitosan-Based Porous Scaffold Into Hyaline-like Cartilage: Study in Heterotopic Implantation
European Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 4-11
Number of views: 2912 Download in PDF
2. Elena Nefed'eva, Tat'yana Veselova, Vladimir Veselovskii, Vladimir LysakEuropean Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 4-11
Abstract:
To study the morphology of remodeling the chitosan-based three-dimensional porous scaffold, containing bone morphogenetic protein-2 (BMP-2) for chondroinduction, the experiments with heterotopic implantation using 28 Wistar rats were carried out. Scaffolds with growth factor (n=12) or without it (n=12), against intact control (n=4) were implanted subcutaneously. Classical methods of histology and morphometry as well as immune histochemical markers (CD-68, CD-31, MMP-9, TIMP-1, and osteonectin expression), one used to investigate zone of remodeling in euthanized animals at 4 and 8 weeks after implantation. The BMP-2 application provides more intensive and rapid new cartilage formation from the scaffold matter. The additional chondroinductive effect proved more intensive settlement and proliferation of chondral cells in the regenerate, expression of chondral phenotype with the building the hyaline-like matrix, and the supporting necessary balance between the matrix metalloproteinases and their tissue inhibitors.
To study the morphology of remodeling the chitosan-based three-dimensional porous scaffold, containing bone morphogenetic protein-2 (BMP-2) for chondroinduction, the experiments with heterotopic implantation using 28 Wistar rats were carried out. Scaffolds with growth factor (n=12) or without it (n=12), against intact control (n=4) were implanted subcutaneously. Classical methods of histology and morphometry as well as immune histochemical markers (CD-68, CD-31, MMP-9, TIMP-1, and osteonectin expression), one used to investigate zone of remodeling in euthanized animals at 4 and 8 weeks after implantation. The BMP-2 application provides more intensive and rapid new cartilage formation from the scaffold matter. The additional chondroinductive effect proved more intensive settlement and proliferation of chondral cells in the regenerate, expression of chondral phenotype with the building the hyaline-like matrix, and the supporting necessary balance between the matrix metalloproteinases and their tissue inhibitors.
Number of views: 2912 Download in PDF
Influence of Pulse Pressure on Seed Quality and Yield of Buckwheat (Fagopyrum esculentum Moench.)
European Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 12-27
Number of views: 2683 Download in PDF
3. Valery V. NovochadovEuropean Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 12-27
Abstract:
A method of pre-sowing treatment of seeds by pulse pressure which is characterized by high intensity and accuracy of dosage has been proposed. Pulse pressures in the range of 11-29 MPa did not lead to acute lethality determined by phosphorescence at room temperature. So there was no elimination of unproductive individuals but pulse pressure stimulated physiological processes in seeds and plants, leading to increased yield. Changes of germination, growth, and distribution of substances as well as plant productivity were observed. Generalization and analysis of experimental results demonstrated zones in dose-dependent area, such as the zone of hormesis (11-17 MPa), transitional zone (20-26 MPa) and stress zone (29 MPa and more).
A method of pre-sowing treatment of seeds by pulse pressure which is characterized by high intensity and accuracy of dosage has been proposed. Pulse pressures in the range of 11-29 MPa did not lead to acute lethality determined by phosphorescence at room temperature. So there was no elimination of unproductive individuals but pulse pressure stimulated physiological processes in seeds and plants, leading to increased yield. Changes of germination, growth, and distribution of substances as well as plant productivity were observed. Generalization and analysis of experimental results demonstrated zones in dose-dependent area, such as the zone of hormesis (11-17 MPa), transitional zone (20-26 MPa) and stress zone (29 MPa and more).
Number of views: 2683 Download in PDF
Growth Factor Technologies in Cartilage Tissue Engineering (Review)
European Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 28-37
Number of views: 2643 Download in PDF
4. Violetta Pavlova, Ekaterina Vasichkina, Elena Nefed'eva, Vladimir LysakEuropean Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 28-37
Abstract:
The article presents a systematic review of literature analyzing the prevalence, base technologies, and perspective directions of growth factor usage in cartilage tissue engineering. The main attention is given to problems of combinations of growth factors in modern scaffolds for cellular settlement and options for mechanical and physical-chemical stimulation of chondrogenesis, including the use of bioreactors.
The article presents a systematic review of literature analyzing the prevalence, base technologies, and perspective directions of growth factor usage in cartilage tissue engineering. The main attention is given to problems of combinations of growth factors in modern scaffolds for cellular settlement and options for mechanical and physical-chemical stimulation of chondrogenesis, including the use of bioreactors.
Number of views: 2643 Download in PDF
Influence of Pulse Pressure on the State of Biopolymers and the Probability of Hydrolysis of Starch in Seeds
European Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 38-44
Number of views: 2795 Download in PDF
5. European Journal of Molecular Biotechnology, 2013, Vol.(1), № 1, pp. 38-44
Abstract:
Damage of seeds which leads to destruction of the crystal lattice and the phase transition of polymers is formed under the pulse pressure (PP) treatment. Biopolymers such as starch compressed under specific conditions can be changed from crystalline to a glassy state; this transition is known to extend the life of seeds. The aging of seeds is involved in the enzymatic glycosylation of proteins and nucleic acids. Reducing sugars which have been produced in seeds by non-enzymatic hydrolysis enter into reaction of glycosylation with proteins and amino acids actively. The authors studied the water absorption by seeds of buckwheat (Fagopyrum esculentum Moench., cultivar Saulyk) treated by PP. The values of PP which were used to treat had an influence on water absorption during the first hours of imbibition. When water content was 60%, hydrolysis of reserve substances could begin, so water potential was created by osmotically active molecules. Gibbs energy calculation by method of groups’ contribution indicated the reduction in probability of starch hydrolysis in plant seeds during transition from the crystalline to the glassy state.
Damage of seeds which leads to destruction of the crystal lattice and the phase transition of polymers is formed under the pulse pressure (PP) treatment. Biopolymers such as starch compressed under specific conditions can be changed from crystalline to a glassy state; this transition is known to extend the life of seeds. The aging of seeds is involved in the enzymatic glycosylation of proteins and nucleic acids. Reducing sugars which have been produced in seeds by non-enzymatic hydrolysis enter into reaction of glycosylation with proteins and amino acids actively. The authors studied the water absorption by seeds of buckwheat (Fagopyrum esculentum Moench., cultivar Saulyk) treated by PP. The values of PP which were used to treat had an influence on water absorption during the first hours of imbibition. When water content was 60%, hydrolysis of reserve substances could begin, so water potential was created by osmotically active molecules. Gibbs energy calculation by method of groups’ contribution indicated the reduction in probability of starch hydrolysis in plant seeds during transition from the crystalline to the glassy state.
Number of views: 2795 Download in PDF