4 December 20, 2016
Articles and Statements
1. Anna N. Berlina, Dmitry V. Sotnikov, Sergei A. Eremin, Liqiang Liu, Chuanlai Xu, Anatoly V. Zherdev
Lateral flow Immunoassay of Sudan I with Direct Calibration Dependence Based on the use of two Kinds of Conjugated Gold Nanoparticles
European Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 117-124.
Number of views: 1848 Download in PDF
2. Valery V. Novochadov, Alexander A. Shiroky, Alexander V. Khopеrskov, Alexander G. LosevEuropean Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 117-124.
Abstract:
A novel lateral flow immunoassay based on the use of two kinds of conjugated gold nanoparticles’ preparations was developed and applied to the detection of the toxic food colorant Sudan I. The gold nanoparticles were conjugated with monoclonal antibodies specific to Sudan and a Sudan-ovalbumin conjugate. Before the immunochromatographic process, the two conjugates were incubated with a tested sample. The results obtained a direct calibration curve for the detection of low-molecular-weight antigen, in contrast to common reverse concentration dependencies. In the absence of the target compound, minimal coloration of the test line was observed. An increase in the Sudan content led to staining and the appearance of a test line, which could be observed by the naked eye. The total duration of the assay is 15 minutes. The visual limit of Sudan I detection was 0.25 ng/mL, and the instrumental limit of detection (LOD) was 7.6 pg/mL. These values were at least three-four points lower than the established permissible level of the contaminant content in foodstuffs. This reserve in sensitivity provides the possibility of working with highly diluted samples and thus excludes the influence of their matrixes on the assay results. The direct dependence of the coloration on the concentration of Sudan I in the proposed assay means it is easy to identify the presence of the contaminant at extremely low concentrations. The common nature of the proposed approach allows its use in lateral flow immunoassays of different compounds.
A novel lateral flow immunoassay based on the use of two kinds of conjugated gold nanoparticles’ preparations was developed and applied to the detection of the toxic food colorant Sudan I. The gold nanoparticles were conjugated with monoclonal antibodies specific to Sudan and a Sudan-ovalbumin conjugate. Before the immunochromatographic process, the two conjugates were incubated with a tested sample. The results obtained a direct calibration curve for the detection of low-molecular-weight antigen, in contrast to common reverse concentration dependencies. In the absence of the target compound, minimal coloration of the test line was observed. An increase in the Sudan content led to staining and the appearance of a test line, which could be observed by the naked eye. The total duration of the assay is 15 minutes. The visual limit of Sudan I detection was 0.25 ng/mL, and the instrumental limit of detection (LOD) was 7.6 pg/mL. These values were at least three-four points lower than the established permissible level of the contaminant content in foodstuffs. This reserve in sensitivity provides the possibility of working with highly diluted samples and thus excludes the influence of their matrixes on the assay results. The direct dependence of the coloration on the concentration of Sudan I in the proposed assay means it is easy to identify the presence of the contaminant at extremely low concentrations. The common nature of the proposed approach allows its use in lateral flow immunoassays of different compounds.
Number of views: 1848 Download in PDF
Comparative Modeling the Thermal Transfer in Tissues with Volume Pathological Focuses and Tissue Engineering Constructs: a Pilot Study
European Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 125-138.
Number of views: 1896 Download in PDF
3. Valery V. Novochadov, Anna S. Krylova, Nikita A. Anikeev, Victor I. Shemonaev, Angelina O. ZekiyEuropean Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 125-138.
Abstract:
The goal of this study was to conduct the computing experiments on the use of the spatial temperature distribution and heat flow in biological tissues obtained on the basis of microwave radiometry, and to develop the predictive algorithm of their specific properties and conditions. In the first stage, the data about the surface and deep temperature in different parts of the mammary glands of healthy women were used to build the model of heat flow in this body, proved that there is a lateral heat transfer from one part to another. Accounting for this phenomenon in the analysis of the spatial distribution of temperature in the breast with verified malignant tumours with installed localization made it possible to prove a several decision rules with a potentially high diagnostic efficiency. Based on the assumption about spatial variation of the dielectric constant and electrical conductivity in biological tissue at the installation site and subsequent remodeling of tissue-engineered constructs to develop in similar patterns, and to be capable of the same impact on brightness temperature of the tissues under the antenna of a microwave radiometer, we conducted simulation in the second phase of the study. The crucial equations, being able to differentiate different states of adaptation (remodeling) of tissue-engineered constructs were offered, that can be used in tissue engineering as creating data structures, also for monitoring postimplantation period.
The goal of this study was to conduct the computing experiments on the use of the spatial temperature distribution and heat flow in biological tissues obtained on the basis of microwave radiometry, and to develop the predictive algorithm of their specific properties and conditions. In the first stage, the data about the surface and deep temperature in different parts of the mammary glands of healthy women were used to build the model of heat flow in this body, proved that there is a lateral heat transfer from one part to another. Accounting for this phenomenon in the analysis of the spatial distribution of temperature in the breast with verified malignant tumours with installed localization made it possible to prove a several decision rules with a potentially high diagnostic efficiency. Based on the assumption about spatial variation of the dielectric constant and electrical conductivity in biological tissue at the installation site and subsequent remodeling of tissue-engineered constructs to develop in similar patterns, and to be capable of the same impact on brightness temperature of the tissues under the antenna of a microwave radiometer, we conducted simulation in the second phase of the study. The crucial equations, being able to differentiate different states of adaptation (remodeling) of tissue-engineered constructs were offered, that can be used in tissue engineering as creating data structures, also for monitoring postimplantation period.
Number of views: 1896 Download in PDF
The Functionalizing Bioactive Surface of Screw Titanium Implants with Chitosan: Fabrication and Surface Features
European Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 139-147.
Number of views: 1838 Download in PDF
4. Alexander S. Semenov, Vladimir V. BulatEuropean Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 139-147.
Abstract:
The necessity to develop different approaches to increasing dental implant osseointegration should be of high prevalence of dental diseases in current world, the massive expansion of dental implantation technologies, and the advent of technical possibilities to functionalize bioactive surfaces using modern molecular biotechnology. To improve bioactive surface of dental implants by functionalization, we selected chitosan because it had the properties of biocompatibility and osteoinductive effect, also we had literature data about the possibility of its application as nano-films and nano-coating. Chitosan, applied using еру cathodic sputtering technique, significantly altered nano-surface topography of dental implants. Using atomic force microscopy it is shown that after chitosan applying, the nano-roughness parameter has 5.6-fold increase, and the developed surface area ratio has increased 3.7 times in comparison with surface properties of commercial titanium screw implant without chitosan spraying. The application of chitosan on bioactive surface of the screw titanium implants was shown to improve the morphological characteristics of osseointegration after implantation into the rat femoral bone. The bone volume fraction in osseointegration zone exceeded at different periods of the experiment the value of the same parameter in the comparison group 1.56-1.64 times. Implants with a surface chitosan-based functional coating provided the additional osteoconductive effect appeared in more intensive and rapid osteogenesis around implants, and the more expressed remodeling and thickening of the surrounding trabecular bone.
The necessity to develop different approaches to increasing dental implant osseointegration should be of high prevalence of dental diseases in current world, the massive expansion of dental implantation technologies, and the advent of technical possibilities to functionalize bioactive surfaces using modern molecular biotechnology. To improve bioactive surface of dental implants by functionalization, we selected chitosan because it had the properties of biocompatibility and osteoinductive effect, also we had literature data about the possibility of its application as nano-films and nano-coating. Chitosan, applied using еру cathodic sputtering technique, significantly altered nano-surface topography of dental implants. Using atomic force microscopy it is shown that after chitosan applying, the nano-roughness parameter has 5.6-fold increase, and the developed surface area ratio has increased 3.7 times in comparison with surface properties of commercial titanium screw implant without chitosan spraying. The application of chitosan on bioactive surface of the screw titanium implants was shown to improve the morphological characteristics of osseointegration after implantation into the rat femoral bone. The bone volume fraction in osseointegration zone exceeded at different periods of the experiment the value of the same parameter in the comparison group 1.56-1.64 times. Implants with a surface chitosan-based functional coating provided the additional osteoconductive effect appeared in more intensive and rapid osteogenesis around implants, and the more expressed remodeling and thickening of the surrounding trabecular bone.
Number of views: 1838 Download in PDF
Ancient Paleo-DNA of Pre-Copper Age North-Eastern Europe: Establishing the Migration Traces of R1a1 Y-DNA Haplogroup Part 2. Baikal Episode and Indo-Uralic Framework
European Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 148-157.
Number of views: 2178 Download in PDF
5. H. A. ShindyEuropean Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 148-157.
Abstract:
The work considers the problems of paleogenetics and anthropology connected with pre-Copper Age after-Glacial repopulation process of the North-Eastern Europe. The unified data, obtained in various laboratories in 2010–2016, collects a certain amount of the ancient mt-DNA and Y-DNA haplogroup samples of the considered period, what allows establishing the connection between some of them. The first part of the paper (Semenov, Bulat, 2016) showed the possible connection of R1a1 Y-haplogroup dispersion in Neolithic time with Comb Ware pottery. The second part shows that the recently found R1a1’s in Neolithic Baikal cultures also fit the hypothesis and can go along with Indo-Uralic language superfamily hypothesis. The paper makes an attempt to build a picture of the population of North-Eastern Europe in pre-Copper Age time and to systemize the pale DNA genotyping results into clusters corresponding to different migration waves. The paper can be of use in biomedical purposes also, as some correlations between diseases and haplogroups were noticed in different medical works.
The work considers the problems of paleogenetics and anthropology connected with pre-Copper Age after-Glacial repopulation process of the North-Eastern Europe. The unified data, obtained in various laboratories in 2010–2016, collects a certain amount of the ancient mt-DNA and Y-DNA haplogroup samples of the considered period, what allows establishing the connection between some of them. The first part of the paper (Semenov, Bulat, 2016) showed the possible connection of R1a1 Y-haplogroup dispersion in Neolithic time with Comb Ware pottery. The second part shows that the recently found R1a1’s in Neolithic Baikal cultures also fit the hypothesis and can go along with Indo-Uralic language superfamily hypothesis. The paper makes an attempt to build a picture of the population of North-Eastern Europe in pre-Copper Age time and to systemize the pale DNA genotyping results into clusters corresponding to different migration waves. The paper can be of use in biomedical purposes also, as some correlations between diseases and haplogroups were noticed in different medical works.
Number of views: 2178 Download in PDF
Characterization, Mechanisms and Applications in the Chemistry of Cyanine Dyes: A Review
European Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 158-170.
Number of views: 2174 Download in PDF
6. European Journal of Molecular Biotechnology, 2016, Vol.(14), Is. 4, pp. 158-170.
Abstract:
In this paper review, some of the important characterizations and mechanisms in the chemistry of cyanine dyes were explained. This include topics like absorption spectral behaviour, general characterization of cyanine dyes, photosensitisation effects of cyanine dyes in silver halide emulsion, photosensitisation-structure correlation of cyanine dyes, colour spectrophotometric measurements of cyanine dyes, fluorescence spectra of cyanine dyes, IR and 1H NMR spectra of cyanine dyes, general view in the visible spectra of cyanine dyes, stability of cyanine dyes, cyanine dyes in photodynamic therapy, synthesis mechanisms of monomethine cyanine dyes and synthesis mechanisms of trimethine cyanine dyes. In addition, in the introduction section of this review paper some light is shed on important uses and applications of cyanine dyes. Reviewing some of the important characterizations and synthesis mechanisms in the chemistry of cyanine dyes is interesting and can be considered as a novel or patent idea to which little attention has been paid in the chemistry literature.
In this paper review, some of the important characterizations and mechanisms in the chemistry of cyanine dyes were explained. This include topics like absorption spectral behaviour, general characterization of cyanine dyes, photosensitisation effects of cyanine dyes in silver halide emulsion, photosensitisation-structure correlation of cyanine dyes, colour spectrophotometric measurements of cyanine dyes, fluorescence spectra of cyanine dyes, IR and 1H NMR spectra of cyanine dyes, general view in the visible spectra of cyanine dyes, stability of cyanine dyes, cyanine dyes in photodynamic therapy, synthesis mechanisms of monomethine cyanine dyes and synthesis mechanisms of trimethine cyanine dyes. In addition, in the introduction section of this review paper some light is shed on important uses and applications of cyanine dyes. Reviewing some of the important characterizations and synthesis mechanisms in the chemistry of cyanine dyes is interesting and can be considered as a novel or patent idea to which little attention has been paid in the chemistry literature.
Number of views: 2174 Download in PDF