1 March 31, 2016
1. Ali Saeed Atiyah AL-Janabi
Molecular Characterization and Genetic Diversity Analysis of Sweet Orange )Citrus sinensis L. Osbeck) Cultivars in Iraq Using RAPD Markers
European Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 4-12.
Number of views: 2444 Download in PDF
2. Ignat Ignatov, Oleg MosinEuropean Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 4-12.
Abstract:
Sweet orange (Citrus sinensis L. Osbeck) is one of the most important commercially cultivated fruit crops of Citrus. Genetic diversity and inter-relationship among 5 cultivars (Indian, Iraqi, Japanese, Syrian, Egyptian) of C. sinensis were analyzed based on RAPD markers. Six primers generated reproducible and easily storable RAPD profiles with a number of amplified DNA fragments ranging from 6 to 14 fragment bands. The total number of amplicons detected was 51, including 14 fragments unique bands with average reached 2.8 fragments ̸ primers. While the number of polymorphic ranged from 0 to 8 with an average reached 4.4 fragments ̸ primers with the polymorphic percentage ranged from 0% to 57.1%. While the number of monomorphic ranged from 2 to 5 fragment bands and was total of the monomorphic 15 fragments with an average reached 3 fragments ̸ primers with the monomorphic percentage was 14.2 % to 83.3%. A maximum numbers of amplicons was amplified with primer OPS-238 reached 14 fragments while the minimum number of fragments was amplified with primer OPS-253 reached 6 fragments. The highest number of polymorphic bands reached 8 fragments was obtained with primer OPS-238 with high percentage 57.1%, while the highest number of monomorphic bands reached 5 fragments with high percentage 83.3% was obtained with primer OPS-253. RAPD markers detected genetic distance and similarity, amaximum genetic distance value was observed between Japanese (Jap) and Syrian (Syr) cultivars reached 0.530 with less similarity value reached 47%, a minimum genetic distance value was observed between sweet Iraqi (Irq) and Indian (Ind) cultivars reached 0.239 with high similarity value reached 76.1%. The similarity matrices were employed in the cluster analysis to generate a dendrogram using the UPGMA method. The cluster tree analysis showed that the sweet orange cultivars were broadly divided into two main groups A and B with similarity reached 50%. A group including individual one cultivars was Japanese. B group was divided into two sub-cluster B1 and B2 with genetic similarity reached 63%. The first sub-cluster (B1) was included two cultivars Iraqi and Indian with high genetic similarity among other cultivars reached 77%. The second sub-cluster (B2) was included two cultivars Egyptian and Syrian with genetic similarity reached 72%.
Sweet orange (Citrus sinensis L. Osbeck) is one of the most important commercially cultivated fruit crops of Citrus. Genetic diversity and inter-relationship among 5 cultivars (Indian, Iraqi, Japanese, Syrian, Egyptian) of C. sinensis were analyzed based on RAPD markers. Six primers generated reproducible and easily storable RAPD profiles with a number of amplified DNA fragments ranging from 6 to 14 fragment bands. The total number of amplicons detected was 51, including 14 fragments unique bands with average reached 2.8 fragments ̸ primers. While the number of polymorphic ranged from 0 to 8 with an average reached 4.4 fragments ̸ primers with the polymorphic percentage ranged from 0% to 57.1%. While the number of monomorphic ranged from 2 to 5 fragment bands and was total of the monomorphic 15 fragments with an average reached 3 fragments ̸ primers with the monomorphic percentage was 14.2 % to 83.3%. A maximum numbers of amplicons was amplified with primer OPS-238 reached 14 fragments while the minimum number of fragments was amplified with primer OPS-253 reached 6 fragments. The highest number of polymorphic bands reached 8 fragments was obtained with primer OPS-238 with high percentage 57.1%, while the highest number of monomorphic bands reached 5 fragments with high percentage 83.3% was obtained with primer OPS-253. RAPD markers detected genetic distance and similarity, amaximum genetic distance value was observed between Japanese (Jap) and Syrian (Syr) cultivars reached 0.530 with less similarity value reached 47%, a minimum genetic distance value was observed between sweet Iraqi (Irq) and Indian (Ind) cultivars reached 0.239 with high similarity value reached 76.1%. The similarity matrices were employed in the cluster analysis to generate a dendrogram using the UPGMA method. The cluster tree analysis showed that the sweet orange cultivars were broadly divided into two main groups A and B with similarity reached 50%. A group including individual one cultivars was Japanese. B group was divided into two sub-cluster B1 and B2 with genetic similarity reached 63%. The first sub-cluster (B1) was included two cultivars Iraqi and Indian with high genetic similarity among other cultivars reached 77%. The second sub-cluster (B2) was included two cultivars Egyptian and Syrian with genetic similarity reached 72%.
Number of views: 2444 Download in PDF
Studying the Composition and Properties of Mountain and Melt Water of Bulgaria and Russia as Factors of Longevity. Effects of Calcium, Magnesium, Zinc and Manganese in Water on Organism
European Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 13-28.
Number of views: 2325 Download in PDF
3. Doncho Krastev, Ignat Ignatov, Oleg Mosin, Penko PenkovEuropean Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 13-28.
Abstract:
This paper demonstrates that mountain and melt water is among the most important factors for longevity. Other factors are hereditary, gender, body weight, food, psychological status, family relationships. Natural waters derived from various Bulgarian water springs, as well as melt water and blood serum of cancer patients between 50 and 70 years old were investigated by IR, NES and DNES-methods. We applied the NES- and DNES-methods for calculation of the average energy of hydrogen bonds (∆EH...O) among H2O molecules in the samples, as well as the percent distribution of H2O molecules according to energies of hydrogen bonds within intervals (from -0.08 to -0.1387 eV). As estimation factor was measured the values of the average energy of hydrogen bonds (∆EH...O) among H2O molecules, as well as local extremums in DNES- and IR-spectra of various samples of water and human blood serum, detected at E = -0.1387 eV and λ = 8.95 μm. For a group of people in critical condition of life and patients with malignant tumors the greatest values of local extremums in DNES-spectra are shifted to lower energies relative to the control group. The IR-spectrum of mountain water is most similar to the IR-spectrum of blood serum of healthy group of people with a local maximum at = 8.95 m. Natural mountain and melt water with unique chemical composition and less deuterium content seems to be one of the most important factors for longevity. In Bulgaria, most long lived people and centenarians live in the Rhodope Mountains, while in Russia – in Dagestan and Yakutia. The similar characteristics possess mountain water from Teteven and other Bulgarian sources. There are new proofs for biophysical and biochemical effects of Ca2+, Mg2+, Zn2+ and Mn2+ in water.
This paper demonstrates that mountain and melt water is among the most important factors for longevity. Other factors are hereditary, gender, body weight, food, psychological status, family relationships. Natural waters derived from various Bulgarian water springs, as well as melt water and blood serum of cancer patients between 50 and 70 years old were investigated by IR, NES and DNES-methods. We applied the NES- and DNES-methods for calculation of the average energy of hydrogen bonds (∆EH...O) among H2O molecules in the samples, as well as the percent distribution of H2O molecules according to energies of hydrogen bonds within intervals (from -0.08 to -0.1387 eV). As estimation factor was measured the values of the average energy of hydrogen bonds (∆EH...O) among H2O molecules, as well as local extremums in DNES- and IR-spectra of various samples of water and human blood serum, detected at E = -0.1387 eV and λ = 8.95 μm. For a group of people in critical condition of life and patients with malignant tumors the greatest values of local extremums in DNES-spectra are shifted to lower energies relative to the control group. The IR-spectrum of mountain water is most similar to the IR-spectrum of blood serum of healthy group of people with a local maximum at = 8.95 m. Natural mountain and melt water with unique chemical composition and less deuterium content seems to be one of the most important factors for longevity. In Bulgaria, most long lived people and centenarians live in the Rhodope Mountains, while in Russia – in Dagestan and Yakutia. The similar characteristics possess mountain water from Teteven and other Bulgarian sources. There are new proofs for biophysical and biochemical effects of Ca2+, Mg2+, Zn2+ and Mn2+ in water.
Number of views: 2325 Download in PDF
Research on the effects of the ‘Dance of the Spiral’ methodology upon the physiological parameters of plants and the essential oil content
European Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 29-39.
Number of views: 2257 Download in PDF
4. Alexander S. Semenov, Vladimir V. BulatEuropean Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 29-39.
Abstract:
“The Dance of the Spiral” is the original methodology consisting of physical exercises that based on ancient health and longevity practices. A research has been done in which this methodology has been applied to plants. Eight plants were chosen including common yarrow, wood violet, dandelion, common chicory, shepherd’s purse, cranesbill, broadleaf plantain and snowdrop, each of them grows the best in one of the eight directions. The planting was performed in eight directions, since each exercise supposedly has an effect on the state of health. Two circles were empirically defined – the Spiral and the Antispiral. Further clinical trials with 20 volunteers suffered from neurological, gastro-intestinal, cardio-vascular, and articular conditions and diseases, who stayed consecutively in the Antispiral and Spiral plant circles for 6 min in each circle were performed based on their subjective assessment of their state of health and their experience after their being stayed in the Antispiral and Spiral circles as a relaxing effect (the Antispiral circle) and a stimulating effect (the Spiral circle). The research involves the composition of essential oil extracts by 1H NMR, gas chromatography and gas chromatography-mass spectrometry as well as the spectral analysis by the methods of NES and DNES of water extracts from the common plant (Achillea millefolium) from the control group growing in its natural habitat and from the plants grown correspondingly in the Spiral and Antispiral circles. The result in the sample of common yarrow from the Spiral circle is an increase of the average energy of hydrogen bonds between H2O molecules. The result in the sample of common yarrow from the Antispiral circle is a decrease of the average energy of hydrogen bonds between H2O molecules. The essential oil composition of the samples planted in the Spiral and Antispiral circles is not identical in the quantitative and qualitative composition regarding the 83 components detected in them. The amount of chamazulene in the control group sample was 5.41 %; in the Spiral sample – 4.32 %; in the Antispiral sample – 10.25 %; i.e. the amount of chamazulene in the essential oil from the Antispiral sample is almost twice of that in the control group and Spiral samples.
“The Dance of the Spiral” is the original methodology consisting of physical exercises that based on ancient health and longevity practices. A research has been done in which this methodology has been applied to plants. Eight plants were chosen including common yarrow, wood violet, dandelion, common chicory, shepherd’s purse, cranesbill, broadleaf plantain and snowdrop, each of them grows the best in one of the eight directions. The planting was performed in eight directions, since each exercise supposedly has an effect on the state of health. Two circles were empirically defined – the Spiral and the Antispiral. Further clinical trials with 20 volunteers suffered from neurological, gastro-intestinal, cardio-vascular, and articular conditions and diseases, who stayed consecutively in the Antispiral and Spiral plant circles for 6 min in each circle were performed based on their subjective assessment of their state of health and their experience after their being stayed in the Antispiral and Spiral circles as a relaxing effect (the Antispiral circle) and a stimulating effect (the Spiral circle). The research involves the composition of essential oil extracts by 1H NMR, gas chromatography and gas chromatography-mass spectrometry as well as the spectral analysis by the methods of NES and DNES of water extracts from the common plant (Achillea millefolium) from the control group growing in its natural habitat and from the plants grown correspondingly in the Spiral and Antispiral circles. The result in the sample of common yarrow from the Spiral circle is an increase of the average energy of hydrogen bonds between H2O molecules. The result in the sample of common yarrow from the Antispiral circle is a decrease of the average energy of hydrogen bonds between H2O molecules. The essential oil composition of the samples planted in the Spiral and Antispiral circles is not identical in the quantitative and qualitative composition regarding the 83 components detected in them. The amount of chamazulene in the control group sample was 5.41 %; in the Spiral sample – 4.32 %; in the Antispiral sample – 10.25 %; i.e. the amount of chamazulene in the essential oil from the Antispiral sample is almost twice of that in the control group and Spiral samples.
Number of views: 2257 Download in PDF
Ancient Paleo-DNA of Pre-Copper Age North-Eastern Europe: Establishing the Migration Traces of R1a1 Y-DNA Haplogroup
European Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 40-54.
Number of views: 2491 Download in PDF
5. European Journal of Molecular Biotechnology, 2016, Vol.(11), Is. 1, pp. 40-54.
Abstract:
The work considers the problems of paleogenetics and anthropology connected with problem of pre-Copper Age after-Glacial repopulation process of the North-Eastern Europe. The unified data, obtained in various laboratories in 2010-2016, collects a certain amount of the ancient mt-DNA and Y-DNA haplogroup samples of the considered period, what allows establishing the connection between some of them, comparing them with the data of neighboring regions, and attributing them to certain migration flows traceable in archeology. The paper makes an attempt to build a picture of the population of North-Eastern Europe in pre-Copper Age time and to systemize the paleo DNA genotyping results into clusters corresponding to different migration waves. The paper can be of use for biomedical purposes also, as some correlations between diseases and haplogroups were noticed in various medical works.
The work considers the problems of paleogenetics and anthropology connected with problem of pre-Copper Age after-Glacial repopulation process of the North-Eastern Europe. The unified data, obtained in various laboratories in 2010-2016, collects a certain amount of the ancient mt-DNA and Y-DNA haplogroup samples of the considered period, what allows establishing the connection between some of them, comparing them with the data of neighboring regions, and attributing them to certain migration flows traceable in archeology. The paper makes an attempt to build a picture of the population of North-Eastern Europe in pre-Copper Age time and to systemize the paleo DNA genotyping results into clusters corresponding to different migration waves. The paper can be of use for biomedical purposes also, as some correlations between diseases and haplogroups were noticed in various medical works.
Number of views: 2491 Download in PDF