Number 1. (in 1 part) September 26, 2014
1. Ignat Ignatov, Oleg Mosin
Studying of Phototransformation of Light Signal by Photoreceptor Pigments - Rhodopsin, Iodopsin and Bacteriorhodopsin
European Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 104-119.
Number of views: 2755 Download in PDF
2. Olga V. Kolotova, Irina V. Vladimtseva, Svetlana N. Orlova, Irina V. Sokolova,European Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 104-119.
Abstract:
This review article views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and their aspects of nano- and biotechnological usage. On an example of bacteriorhodopsin is described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, the solubilization with 0,5 % (w/v) SDS-Na and subsequent fractionation by methanol and gel filtration chromatography on Sephadex G-200 Column balanced with 0.09 M Tris-borate buffer (pH = 8,35) with 0,1 % (w/v) SDS-Na and 2,5 mM EDTA. Within the framework of the research the mechanism of color perception by the visual analyzer having the ability to analyze certain ranges of the optical spectrum, as colors was studied along with an analysis of the additive mixing of two colors. It was shown that at the mixing of electromagnetic waves with different wavelengths, the visual analyzer perceive them as separate or average wave length corresponding to mix color.
This review article views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and their aspects of nano- and biotechnological usage. On an example of bacteriorhodopsin is described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, the solubilization with 0,5 % (w/v) SDS-Na and subsequent fractionation by methanol and gel filtration chromatography on Sephadex G-200 Column balanced with 0.09 M Tris-borate buffer (pH = 8,35) with 0,1 % (w/v) SDS-Na and 2,5 mM EDTA. Within the framework of the research the mechanism of color perception by the visual analyzer having the ability to analyze certain ranges of the optical spectrum, as colors was studied along with an analysis of the additive mixing of two colors. It was shown that at the mixing of electromagnetic waves with different wavelengths, the visual analyzer perceive them as separate or average wave length corresponding to mix color.
Number of views: 2755 Download in PDF
The Research of Growth Capability Intensification and Lipolytic Activity of Fat Splitting Microbial Cultures by Influence of Natural Mineral Components
European Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 120-130.
Number of views: 2542 Download in PDF
3. Oleg Mosin, Ignat IgnatovEuropean Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 120-130.
Abstract:
23 bacterial strains with lipase activity were separated from the waste water of the meat-processing enterprises and Volgograd water-handling facilities aeration tank. There were studied their morphological, cultural and biochemical properties and their animal and vegetable fat splitting ability. Comparative analysis of fat splitting ability has been carried out for searched strains and devices of enzyme activity increasing and growing process intensity of searched microorganisms were investigated. Mineral nature ingredients – the lake Elton brine, the bischofite, the Dead Sea salt – were added in the culture mediums for increasing of biomass accumulation. It has been investigated that addition of the brine into the culture medium in 5 % concentration increases the growth rate of producer lipase on 293 % in comparison with growth of the control medium without the brine. Addition of 0,25 % the Dead Sea salt concentration intensifies the growth on 317 % in comparison with the control medium. Laboratory simulation of fat waste water purification process has been realized with one of separated activity bacterial strains and it has been showed that addition of the 0,5 % bishofite into the fermenter stimulates lipase activity of the searched microorganisms.
23 bacterial strains with lipase activity were separated from the waste water of the meat-processing enterprises and Volgograd water-handling facilities aeration tank. There were studied their morphological, cultural and biochemical properties and their animal and vegetable fat splitting ability. Comparative analysis of fat splitting ability has been carried out for searched strains and devices of enzyme activity increasing and growing process intensity of searched microorganisms were investigated. Mineral nature ingredients – the lake Elton brine, the bischofite, the Dead Sea salt – were added in the culture mediums for increasing of biomass accumulation. It has been investigated that addition of the brine into the culture medium in 5 % concentration increases the growth rate of producer lipase on 293 % in comparison with growth of the control medium without the brine. Addition of 0,25 % the Dead Sea salt concentration intensifies the growth on 317 % in comparison with the control medium. Laboratory simulation of fat waste water purification process has been realized with one of separated activity bacterial strains and it has been showed that addition of the 0,5 % bishofite into the fermenter stimulates lipase activity of the searched microorganisms.
Number of views: 2542 Download in PDF
Evolution, Metabolism and Biotechnological Usage of Methylotrophic Microorganisms
European Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 131-148.
Number of views: 2700 Download in PDF
4. Swati Tyagi, Ramesh Singh, Shaily JaveriaEuropean Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 131-148.
Abstract:
Methylotrophs – aerobic chemoheterotrophic microorganisms submitted by cocci and bacilli mobile forms, are inhabitants of reservoirs and soils of various type, where there are going on various processes of decomposition of organic substances with formation of the one-carbon С1-compounds and some С2-, and С3-compounds, capable to be assimilated by methylotrophs. These microorganisms assimilating carbon on ribuloso-5-monophospate and serine pathways, are allocated from soil ground, the sewage containing decomposing vegetative remainss, from ruminant paunch and other sources. Methylotrophic bacteria recently draw the increasing attention of biotechnology as feasible sources of natural biologically active compounds – fodder fibers and irreplaceable amino acids, carotenoid pigments, lipids and policarbohydrates. For preparation of these compounds are used genetically marked strains of methylotrophic bacteria, obtained via genetic engineering approaches and selection. Recently developed gene-engineering methods of manipulation with the methylotrophic genom allow create on the basis of microbic DNA of methylotrophs expression vectors of eukaryotic proteins for medical and veterinary purposes, as human insulins. In this review article there are submitted data including the results of the authors’ own research on evolution of methylotrophic bacteria, the metabolism and their biotechnological usage.
Methylotrophs – aerobic chemoheterotrophic microorganisms submitted by cocci and bacilli mobile forms, are inhabitants of reservoirs and soils of various type, where there are going on various processes of decomposition of organic substances with formation of the one-carbon С1-compounds and some С2-, and С3-compounds, capable to be assimilated by methylotrophs. These microorganisms assimilating carbon on ribuloso-5-monophospate and serine pathways, are allocated from soil ground, the sewage containing decomposing vegetative remainss, from ruminant paunch and other sources. Methylotrophic bacteria recently draw the increasing attention of biotechnology as feasible sources of natural biologically active compounds – fodder fibers and irreplaceable amino acids, carotenoid pigments, lipids and policarbohydrates. For preparation of these compounds are used genetically marked strains of methylotrophic bacteria, obtained via genetic engineering approaches and selection. Recently developed gene-engineering methods of manipulation with the methylotrophic genom allow create on the basis of microbic DNA of methylotrophs expression vectors of eukaryotic proteins for medical and veterinary purposes, as human insulins. In this review article there are submitted data including the results of the authors’ own research on evolution of methylotrophic bacteria, the metabolism and their biotechnological usage.
Number of views: 2700 Download in PDF
Effect of Climate Change on Plant-Microbe Interaction: An Overview
European Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 149-156.
Number of views: 3262 Download in PDF
5. European Journal of Molecular Biotechnology, 2014, Vol.(5), № 3, pp. 149-156.
Abstract:
Climate change is one of the major issues affecting all of us on our planet. Predicted increase in temperature and decrease in precipitation due to climate change may add complexity and uncertainty to plant and agricultural systems and threaten their sustainable management. It is well known that beneficial plant-associated microorganisms may stimulate plant growth and enhance resistance to disease and abiotic stresses. Climate change will also influence crop quality and the dynamics of the relationships between pests/diseases and crops. Changes in climatic factors like temperature, solar radiation and precipitation have potentials to influence crop production. This now makes it possible to test whether some general patterns occur and whether different groups of plant-associated microorganisms respond differently or in the same way to climate change. Here, we review and discuss how the climatic parameters including atmospheric CO2 and temperature influence the plant–microbe interaction in polluted soils. This review shows that predicting how plant–microbes interaction responds to altering climatic change is critical to select suitable crop plants that would be able to produce more yields and may tolerate multi-stress conditions.
Climate change is one of the major issues affecting all of us on our planet. Predicted increase in temperature and decrease in precipitation due to climate change may add complexity and uncertainty to plant and agricultural systems and threaten their sustainable management. It is well known that beneficial plant-associated microorganisms may stimulate plant growth and enhance resistance to disease and abiotic stresses. Climate change will also influence crop quality and the dynamics of the relationships between pests/diseases and crops. Changes in climatic factors like temperature, solar radiation and precipitation have potentials to influence crop production. This now makes it possible to test whether some general patterns occur and whether different groups of plant-associated microorganisms respond differently or in the same way to climate change. Here, we review and discuss how the climatic parameters including atmospheric CO2 and temperature influence the plant–microbe interaction in polluted soils. This review shows that predicting how plant–microbes interaction responds to altering climatic change is critical to select suitable crop plants that would be able to produce more yields and may tolerate multi-stress conditions.
Number of views: 3262 Download in PDF