Number 1. (in 1 part) March 16, 2014
1. Pavel A. Krylov
The Grouping of Chondrocyte Receptors According to Their Control over Cartilage Tissue Remodeling
European Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 4-10
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2. Oleg Mosin, Ignat IgnatovEuropean Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 4-10
Abstract:
To give the empirical space for development of molecular biotechnologies in regenerative medicine the article presents a systematic review of new data for the last 5 years, dedicated to chondrocyte receptor apparatus, connected to physiological and reparative regeneration in articular cartilage. The main attention is given to the receptors of chondrocytes involved in synthesis and degradation extracellular matrix, differentiation and apoptosis of chondrocytes.
To give the empirical space for development of molecular biotechnologies in regenerative medicine the article presents a systematic review of new data for the last 5 years, dedicated to chondrocyte receptor apparatus, connected to physiological and reparative regeneration in articular cartilage. The main attention is given to the receptors of chondrocytes involved in synthesis and degradation extracellular matrix, differentiation and apoptosis of chondrocytes.
Number of views: 2634 Download in PDF
Biological Influence of Deuterium on Procariotic and Eukaryotic Cells
European Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 11-24
Number of views: 2788 Download in PDF
3. Oleg Mosin, Ignat Ignatov, Dmitry Skladnev, Vitaly ShvetsEuropean Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 11-24
Abstract:
Biologic influence of deuterium (D) on cells of various taxonomic groups of prokaryotic and eukaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photo-organotrophic, and photosynthetic ways of assimilation of carbon substrates are investigated at growth on media with heavy water (D2О). The method of step by step adaptation technique of cells to D2О was developed, consisting in plating of cells on 2 % agarose nutrient media containing increasing gradient of concentration of D2О (from 0 up to 98 % D2O) and the subsequent selection of stable to D2O cells. In the result of that technique were obtained adapted to maximum concentration of D2O cells, biological material of which instead of hydrogen contained deuterium with levels of enrichment 92–97,5 at.% D.
Biologic influence of deuterium (D) on cells of various taxonomic groups of prokaryotic and eukaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photo-organotrophic, and photosynthetic ways of assimilation of carbon substrates are investigated at growth on media with heavy water (D2О). The method of step by step adaptation technique of cells to D2О was developed, consisting in plating of cells on 2 % agarose nutrient media containing increasing gradient of concentration of D2О (from 0 up to 98 % D2O) and the subsequent selection of stable to D2O cells. In the result of that technique were obtained adapted to maximum concentration of D2O cells, biological material of which instead of hydrogen contained deuterium with levels of enrichment 92–97,5 at.% D.
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Using of Facultative Methylotrophic Bacterium Brevibacterium Methylicum В-5652 With RMP-cycle of Carbon Assimilation for Microbiological Synthesis of [2H]phenylalanine With Different Levels of Deuterium Enrichment
European Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 25-40
Number of views: 2748 Download in PDF
4. Ildar G. ShaikhievEuropean Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 25-40
Abstract:
With using of a strain of of L-phenylalanine secreted Gram-positive aerobic facultative methylotrophic bacteria Brevibacterium methylicum В-5652, assimilating methanol via ribulose-5-monophosphate (RMP) cycle of carbon assimilation it was carried out the preparative microbiological synthesis of phenylalanine and metabolically related amino acids (alanine, valine, leucine/isoleucine in the amount of 5–6 mmol/l), labelled with deuterium (2H). The data on adaptation of L-phenylalanine secreted methylotrophic bacterium В. methylicum to the maximal concentration of deuterium in minimal growth medium M9 with 98 % 2Н2O and 2 % [2Н]methanol, and data on biosynthesis of deuterium labelled L-phenylalanine with different levels of enrichment are submitted. The developed method for biosynthesis allows to obtain [2Н]phenylalanine with different levels of isotopic enrichment, depending on 2Н2O concentration in growth media M9, from 17 % (2 deuterium atoms) (on growth medium with 24,5 % 2Н2О) right up to 75 % (6 deuterium atoms) (on growth medium with 98 % 2Н2О) with introduction of deuterium to benzyl С6Н5СН2-fragment of molecule that is confirmed with the data of the electron impact (EI) mass-spectrometry analysis of methyl ethers of N-dimethylamino(naphthalene)-5-sulfochloride (dansyl) derivatives of [2Н]phenylalanine, isolated from the liquid culture after its separation by RP HPLC.
With using of a strain of of L-phenylalanine secreted Gram-positive aerobic facultative methylotrophic bacteria Brevibacterium methylicum В-5652, assimilating methanol via ribulose-5-monophosphate (RMP) cycle of carbon assimilation it was carried out the preparative microbiological synthesis of phenylalanine and metabolically related amino acids (alanine, valine, leucine/isoleucine in the amount of 5–6 mmol/l), labelled with deuterium (2H). The data on adaptation of L-phenylalanine secreted methylotrophic bacterium В. methylicum to the maximal concentration of deuterium in minimal growth medium M9 with 98 % 2Н2O and 2 % [2Н]methanol, and data on biosynthesis of deuterium labelled L-phenylalanine with different levels of enrichment are submitted. The developed method for biosynthesis allows to obtain [2Н]phenylalanine with different levels of isotopic enrichment, depending on 2Н2O concentration in growth media M9, from 17 % (2 deuterium atoms) (on growth medium with 24,5 % 2Н2О) right up to 75 % (6 deuterium atoms) (on growth medium with 98 % 2Н2О) with introduction of deuterium to benzyl С6Н5СН2-fragment of molecule that is confirmed with the data of the electron impact (EI) mass-spectrometry analysis of methyl ethers of N-dimethylamino(naphthalene)-5-sulfochloride (dansyl) derivatives of [2Н]phenylalanine, isolated from the liquid culture after its separation by RP HPLC.
Number of views: 2748 Download in PDF
Linen Fire as Biosorbent to Remove Heavy Metal Ions From Wastewater Modeling
European Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 41-48
Number of views: 2722 Download in PDF
5. European Journal of Molecular Biotechnology, 2014, Vol.(3), № 1. pp. 41-48
Abstract:
The possibility of using linen fires – lnopererabotki waste as a sorption material for the extraction of heavy metal ions from wastewater modeling. It is shown that treatment with acid solutions linen fires a low concentration increases the surface area of linen fires and thus sorption capacity for heavy metal ions. The values of the maximum sorption capacity ions Fe (III), Co (II), Ni (II) and Zn (II) under static and dynamic conditions. IR spectroscopy shows that along with the physical sorption process proceeds chemisorption.
The possibility of using linen fires – lnopererabotki waste as a sorption material for the extraction of heavy metal ions from wastewater modeling. It is shown that treatment with acid solutions linen fires a low concentration increases the surface area of linen fires and thus sorption capacity for heavy metal ions. The values of the maximum sorption capacity ions Fe (III), Co (II), Ni (II) and Zn (II) under static and dynamic conditions. IR spectroscopy shows that along with the physical sorption process proceeds chemisorption.
Number of views: 2722 Download in PDF