Number 1. (in 1 part) December 20, 2013
1. Dmitry A. Antonov
Molecular Mechanisms of the Lubrucating Function of the Synovial Fluid Control
European Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 48-57
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2. Dmitry M. Frolov, Valery G. ZaitsevEuropean Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 48-57
Abstract:
The author in his review presents up-to-date information, concerning the composition and properties of human synovial fluid with an emphasis on detailed description of the molecular mechanisms of the lubricating function control. Three main groups of molecules: (surface-active phospholipids (1), hyaluronic acid (2), and proteins group SZP/lubricine (3), were described in synovial fluid being able to support lubrication; the latter group was considered as the main biopolymers, ensuring the boundary lubrication. On author’s opinion, the modern practice of viscosupplementation is not fully functional, since it did not restore the function of the boundary lubrication, lost in the osteoarthritis progression. Besides the need to correct the composition of current drugs for viscosupplementary therapy, the ensuring of lubricating properties of tissue engineering constructs and media to grow cartilage tissue was in vitro attributed to the practical tasks of joints regenerative biomedicine.
The author in his review presents up-to-date information, concerning the composition and properties of human synovial fluid with an emphasis on detailed description of the molecular mechanisms of the lubricating function control. Three main groups of molecules: (surface-active phospholipids (1), hyaluronic acid (2), and proteins group SZP/lubricine (3), were described in synovial fluid being able to support lubrication; the latter group was considered as the main biopolymers, ensuring the boundary lubrication. On author’s opinion, the modern practice of viscosupplementation is not fully functional, since it did not restore the function of the boundary lubrication, lost in the osteoarthritis progression. Besides the need to correct the composition of current drugs for viscosupplementary therapy, the ensuring of lubricating properties of tissue engineering constructs and media to grow cartilage tissue was in vitro attributed to the practical tasks of joints regenerative biomedicine.
Number of views: 2608 Download in PDF
Chitosan-based Matrix, Used to Determine the Bacterial Lipopolysaccharide in Air
European Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 58-62
Number of views: 2628 Download in PDF
3. Oleg Mosin, Ignat Ignatov, Dmitry Skladnev, Vitaly ShvetsEuropean Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 58-62
Abstract:
The article describes the technology of chitosan-based matrix creation, and results of the study of its affine properties to bacterial lipopolysaccharide in aerosol dispersion. High degree of deacylation of polymer (over 97%), three-dimensional-porous structure, and multilayer packaging in analytical cartridge were the features of this matrix. Specified air volume, containing aerosol concentration of bacterial lipopolysaccharide, was passed through the glass cylinder with analytical container. The share of captured molecules ranged from 1.0% to 1.5%, demonstrating the efficiency of chitosan matrix. It is suitable for the creation of the devices for bacterial lipopolysaccharide detection in the air, based on the obtained matrix.
The article describes the technology of chitosan-based matrix creation, and results of the study of its affine properties to bacterial lipopolysaccharide in aerosol dispersion. High degree of deacylation of polymer (over 97%), three-dimensional-porous structure, and multilayer packaging in analytical cartridge were the features of this matrix. Specified air volume, containing aerosol concentration of bacterial lipopolysaccharide, was passed through the glass cylinder with analytical container. The share of captured molecules ranged from 1.0% to 1.5%, demonstrating the efficiency of chitosan matrix. It is suitable for the creation of the devices for bacterial lipopolysaccharide detection in the air, based on the obtained matrix.
Number of views: 2628 Download in PDF
Use of Gram-positive Chemoheterotrophic Bacterium Basillus subtilis В-3157 with HMP-cycle of Carbon Assimilation for Microbiological Synthesis of [2H]riboxine with High Level of Deuterium Enrichment
European Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 63-78
Number of views: 2947 Download in PDF
4. Violetta Pavlova, Ekaterina Vasichkina, Sergey Belopuhov, Alexey Kolotvin, Vladimir LysakEuropean Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 63-78
Abstract:
We studied the growth and biosynthetic properties of a strain of Gram-positive chemoheterotriphic bacterium Bacillus subtilis В-3157 producer of 2H-labeled purine ribonucleoside riboxine (outcome is 3,9 g/l) in heavy hydrogen (HH) medium with high level of deuterium enrichment (99,8 at.% 2H2O) with 2 % hydrolysate of deuterated biomass of methylotrophic bacterium Brevibacterium methylicum B-5662 as a source of 2H-labeled growth substrates, obtained in the minimal М9 growth medium of 98 % 2Н2О and 2 % [2H]methanol. Isolation of riboxine from liquid culture of riboxine producer strain was carried out by adsorption/resorption on a surface of activated carbon coal, extraction by 0.3 M NH4-formiate buffer (рН = 8.9) with the subsequent crystallization in 80 % of ethanol and column ion exchange chromatography on cation exchanger AG50WX 4, counterbalanced with 0.3 M NH4-formiate with 0.045 M NH4Cl (outcome of riboxine is 3.1 g/l (80 %)). The level of deuterium enrichment of biosynthetically prepared riboxine, analyzed by a method of fast atom bombardment (FAB) mass-spectrometry makes up 5 deuterium atoms with incorporation of 3 deuterium atoms into ribose and 2 deuterium atoms into hypoxantine fragments of the molecule.
We studied the growth and biosynthetic properties of a strain of Gram-positive chemoheterotriphic bacterium Bacillus subtilis В-3157 producer of 2H-labeled purine ribonucleoside riboxine (outcome is 3,9 g/l) in heavy hydrogen (HH) medium with high level of deuterium enrichment (99,8 at.% 2H2O) with 2 % hydrolysate of deuterated biomass of methylotrophic bacterium Brevibacterium methylicum B-5662 as a source of 2H-labeled growth substrates, obtained in the minimal М9 growth medium of 98 % 2Н2О and 2 % [2H]methanol. Isolation of riboxine from liquid culture of riboxine producer strain was carried out by adsorption/resorption on a surface of activated carbon coal, extraction by 0.3 M NH4-formiate buffer (рН = 8.9) with the subsequent crystallization in 80 % of ethanol and column ion exchange chromatography on cation exchanger AG50WX 4, counterbalanced with 0.3 M NH4-formiate with 0.045 M NH4Cl (outcome of riboxine is 3.1 g/l (80 %)). The level of deuterium enrichment of biosynthetically prepared riboxine, analyzed by a method of fast atom bombardment (FAB) mass-spectrometry makes up 5 deuterium atoms with incorporation of 3 deuterium atoms into ribose and 2 deuterium atoms into hypoxantine fragments of the molecule.
Number of views: 2947 Download in PDF
Effect of Pulse Pressure Treatment on Content of Protein and Some Sugars in Wheat Seeds
European Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 79-84
Number of views: 2685 Download in PDF
5. European Journal of Molecular Biotechnology, 2013, Vol.(2), № 2, pp. 79-84
Abstract:
Seeds of hard wheat (Triticum durum) were treated by pulse pressure (PP), generated by shock wave. Protein content was determined by spectroscopy in the nearest infrared point of the spectrum (NIR). HPLC was used for determining glucose level in samples. Moisture of control seeds was 14.2%. It was not changed in PP 11 MPa samples and was decreased by PP 29 MPa (12.8%); that indicated the damage of seeds. Protein content in control sample was 16.5%. The index was not changed by PP 11 MPa (16.9%), but it increased under PP 29 MPa (17.2%) due to the degradation of starch and relative elevation of protein level. Glucose content in PP 11 MPa samples was less than the control level, probably, due to the acceleration of amino-carbonyl reaction. PP 29 MPa promoted the increase of glucose content due to non-enzymatic starch hydrolysis activation. Thus PP 11 MPa made minor damages without the accumulation of glucose, but PP 29 MPa promoted the ageing due to the accumulation of glucose.
Seeds of hard wheat (Triticum durum) were treated by pulse pressure (PP), generated by shock wave. Protein content was determined by spectroscopy in the nearest infrared point of the spectrum (NIR). HPLC was used for determining glucose level in samples. Moisture of control seeds was 14.2%. It was not changed in PP 11 MPa samples and was decreased by PP 29 MPa (12.8%); that indicated the damage of seeds. Protein content in control sample was 16.5%. The index was not changed by PP 11 MPa (16.9%), but it increased under PP 29 MPa (17.2%) due to the degradation of starch and relative elevation of protein level. Glucose content in PP 11 MPa samples was less than the control level, probably, due to the acceleration of amino-carbonyl reaction. PP 29 MPa promoted the increase of glucose content due to non-enzymatic starch hydrolysis activation. Thus PP 11 MPa made minor damages without the accumulation of glucose, but PP 29 MPa promoted the ageing due to the accumulation of glucose.
Number of views: 2685 Download in PDF